单项选择题
The appropriate ligand is attached to the matrix of the column and a protein extract is run through the column. The only macromolecule that will attach to the column will be the one that binds to the ligand; all the other macromolecules will wash through the column. The column is then treated in such a manner that the ligand and the macromolecule will dissociate and the macromolecule will then elute off. Elution by inclusion of free ligand in buffer is common. Other methods of elution can be
A.lowering the pH of buffer
B.increasing or decreasing salt concentrations
C.decreasing the temperature of the column
D.all of these